Year

2018

Type(s)

Journal Article

Author(s)

Morawska, Kamila and Popławski, Tomasz and Ciesielski, Witold and Smarzewska, Sylwia

Source

Bioelectrochemistry, 123: 227—232, 2018

Url

http://www.sciencedirect.com/science/article/pii/S156753941730659X

BibTeX

BibTeX

BibTeX

@article{morawska_electrochemical_2018, title = {Electrochemical and spectroscopic studies of the interaction of antiviral drug {Tenofovir} with single and double stranded {DNA}}, volume = {123}, issn = {1567-5394}, url = {http://www.sciencedirect.com/science/article/pii/S156753941730659X}, doi = {10.1016/j.bioelechem.2018.06.002}, abstract = {In the present study, the electrochemical behavior of the antiviral drug tenofovir (tnf) on a boron-doped diamond electrode (BDDE) has been studied using square-wave voltammetry (SWV). The experimental conditions such as the supporting electrolyte composition and the SWV parameters were optimized. Under the optimized conditions, a simple and sensitive SWV procedure for tnf determination was developed in the concentration range of 5.0 × 10−6 to 1.0 × 10−4 mol L−1. The calculated limit of detection and limit of quantification were equal to 5.6 × 10−7 and 1.9 × 10−6 mol L−1, respectively. The biological significance of the developed method was demonstrated by a quantitative analysis of the pharmaceutical formulations Viread and Tenofovir disoproxil Teva. Moreover, both voltammetric and spectroscopic techniques were used to study the interaction between tnf and DNA. Changes in the electrochemical and spectroscopic behavior of tnf in the presence of DNA were used to calculate the binding constants of the formed complexes. The estimated values of Gibbs free energy revealed that the formation of the drug–DNA complexes was a spontaneous and favorable process. Moreover, for free and bound tenofovir, kinetic parameters such as heterogeneous rate constant, electron transfer coefficient, and diffusion coefficient were determined.}, urldate = {2018-09-11}, journal = {Bioelectrochemistry}, author = {Morawska, Kamila and Popławski, Tomasz and Ciesielski, Witold and Smarzewska, Sylwia}, month = oct, year = {2018}, keywords = {Boron doped diamond, dsDNA, ssDNA, Tenofovir, Voltammetry}, pages = {227--232}, file = {ScienceDirect Full Text PDF:C:\Users\Tomasz Poplawski\Zotero\storage\B7VBAVMU\Morawska i in. - 2018 - Electrochemical and spectroscopic studies of the i.pdf:application/pdf;ScienceDirect Snapshot:C:\Users\Tomasz Poplawski\Zotero\storage\KPRR4KKP\S156753941730659X.html:text/html}