Year

2012

Type(s)

Journal Article

Author(s)

Szczepanska, Joanna and Poplawski, Tomasz and Synowiec, Ewelina and Pawlowska, Elzbieta and Chojnacki, Cezary J. and Chojnacki, Jan and Blasiak, Janusz

Source

Molecular Biology Reports, 39(2): 1561—1574, 2012

BibTeX

BibTeX

BibTeX

@article{szczepanska_2-hydroxylethyl_2012, title = {2-hydroxylethyl methacrylate ({HEMA}), a tooth restoration component, exerts its genotoxic effects in human gingival fibroblasts trough methacrylic acid, an immediate product of its degradation}, volume = {39}, issn = {1573-4978}, doi = {10.1007/s11033-011-0895-y}, abstract = {HEMA (2-hydroxyethyl methacrylate), a methacrylate commonly used in dentistry, was reported to induce genotoxic effects, but their mechanism is not fully understood. HEMA may be degraded by the oral cavity esterases or through mechanical stress following the chewing process. Methacrylic acid (MAA) is the primary product of HEMA degradation. In the present work we compared cytotoxic and genotoxic effects induced by HEMA and MAA in human gingival fibroblasts (HGFs). A 6-h exposure to HEMA or MAA induced a weak decrease in the viability of HGFs. Neither HEMA nor MAA induced strand breaks in the isolated plasmid DNA, but both compounds evoked DNA damage in HGFs, as evaluated by the alkaline comet assay. Oxidative modifications to the DNA bases were monitored by the DNA repair enzymes Endo III and Fpg. DNA damage induced by HEMA and MAA was not persistent and was removed during a 120 min repair incubation. Results from the neutral comet assay indicated that both compounds induced DNA double strand breaks (DSBs) and they were confirmed by the γ-H2AX assay. Both compounds induced apoptosis and perturbed the cell cycle. Therefore, methacrylic acid, a product of HEMA degradation, may be involved in its cytotoxic and genotoxic action.}, language = {eng}, number = {2}, journal = {Molecular Biology Reports}, author = {Szczepanska, Joanna and Poplawski, Tomasz and Synowiec, Ewelina and Pawlowska, Elzbieta and Chojnacki, Cezary J. and Chojnacki, Jan and Blasiak, Janusz}, month = feb, year = {2012}, pmid = {21617943}, pmcid = {PMC3249584}, keywords = {Humans, Flow Cytometry, Apoptosis, Statistics, Nonparametric, Dental Materials, Methacrylates, Mutagens, DNA Breaks, Double-Stranded, Cell Line, Fibroblasts, Gingiva, Molecular Structure, comet assay}, pages = {1561--1574}